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KMID : 0861020080230010109
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Korea Journal of Herbology
2008 Volume.23 No. 1 p.109 ~ p.116
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A Study on Discriminative Criteria of 6 Kinds of Achyranthis Radix Using HPLC/DAD Isolation and Identification of 20-hydroxyecdysone from Achyranthes japonica NAKAI and Comparison of Patterns of Achyranthis Radix from Different Locations by HPLC
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Kim Jeong-Hee
Kim Jong-Mun
Kang Dae-Hoon
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Abstract
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Objectives: This study was performed to investigate the discriminative criteria of 6 kinds of Achyranthis Radix by HPLC/DAD.
Methods: 20-hydroxyecdysone is isolated by silica gel column chromatography (CHCl3:MeOH, 7:1-1:1
v/v) and identified by nuclear magnetic resonance. A high-performance liquid chromatographic method
with diode array detection was used to identify 20-hydroxyecdysone in A. japonica. The analysis was
performed using C18 column with isocratic elution consisted of 18% acetonitrile and 82% water and the
detection was carried out by DAD at 254 nm. 6 kinds of Achyranthis Radix from different locations
were extracted in MeOH. Each extracts was analyzed by HPLC in same condition as used in analysis of
20-hydroxyecdysone. The identities of each extracts were determined by comparing the retention time
and UV spectrum with that of reference compound.
Results: 1. A. japonica and A. bidentata showed the similar patterns of HPLC chromatogram and 20-hydroxycedysone was present in both of them because the peaks having the same retention time and UV spectrum as 20-hydroxyecdysone were shown in the HPLC chromatograms of A. japonica and A. bidentata 2. Cyathula officinalis and C. capitata showed the similar patterns of HPLC chromatogram. The peak having the same retention time and UV spectrum as 20-hydroxyecdysone was shown in the HPLC chromatogram of C. capitata but not shown in the HPLC chromatogram of C. officinalis. 3. Two species of medicinal drugs from Sacheon province showed similar patterns of HPLC chromatogram. Achyranthis Radix from Sacheon(wild) did not have 20-hydroxycedysone but Achyranthis Radix from
Sacheon(cultivated) showed the peak having the same retention time as 20-hydroxyecdysone but UV
spectrum of the peak was different from that of 20-hydroxyecdysone.
Conclusions: These results suggested that 20-hydroxyecdysone could be the discriminative criteria for
Achyranthis Radix contain 20-hydroxyecdysone though they belong to different genus and species. And
the patterns of HPLC chromatogram also could be the discriminative criteria as the different species of
Achyranthis Radix belonging to the same genus showed similar patterns of HPLC chromatogram
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KEYWORD
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NMR, HPLC/DAD, 20-hydroxyecdysone, Cyathula, Achyranthes
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